Hplc Column
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 Phenomenex Chirex S LEU 250x8mm Micron HPLC Column $90.00 Time Remaining: 29d 14h 1m Buy It Now for only: $90.00 |
 Phenomenex HPLC column Synergi 4u Fusion RP 80A 00F 4424 E0 size 150 X 46 mm $25.00 (1 Bid) Time Remaining: 2d 9h 51m |
 HPLC Column Phenomenex Jupiter 4u Proteo 90A $272.25 Time Remaining: 28d 13h 58m Buy It Now for only: $272.25 |
 Supelco Discovery C18 HPLC Column 245cm x 46mm 5m $24.95 Time Remaining: 17h 46m |
 Hewlett Packard ODS Hypersil 3UM HPLC Column $40.00 Time Remaining: 16d 12h 13m Buy It Now for only: $40.00 |
 Phenomenex LUNA 5u C18 250x460mm HPLC Column $90.00 Time Remaining: 29d 14h 9m Buy It Now for only: $90.00 |
 SIGMA SUPELCO SUPELCOSIL LC 18 DB 75cm X 30mm 3um 120 HPLC COLUMN $49.95 Time Remaining: 4d 17h 48m |
 Phenomenex IB SIL 5 C18 HPLC Column 50 x 32mm $49.99 Time Remaining: 18d 10h 45m Buy It Now for only: $49.99 |
 HPLC column Supelco 250 mm $20.00 Time Remaining: 15d 16h 49m Buy It Now for only: $20.00 |
 PERKIN ELMER 0711 0077 220X46mm ION EXCHANGE AX 300 7um HPLC COLUMN $49.95 Time Remaining: 4d 17h 48m |
 HPLC Column Agilent Zobrax SB C18 $273.24 Time Remaining: 28d 13h 58m Buy It Now for only: $273.24 |
 HPLC column Shandon Hypersil ODS 5um 46x250mm $33.00 Time Remaining: 18d 23h Buy It Now for only: $33.00 |
 Eppendorf CH 30 HPLC Column Heater + TC 50 Temperature Controller $279.00 Time Remaining: 6d 11h 23m Buy It Now for only: $319.00 |
 HPLC column ES Chromegabond MC 18 80A 5um 46x250mm $33.00 Time Remaining: 17d 20h 58m Buy It Now for only: $33.00 |
 HPLC column Vydac Protein C4 50mm $17.00 Time Remaining: 19d 16h 40m Buy It Now for only: $17.00 |
 HIGGINS ANALYTICAL HF 2246 W085 HAISIL 300 C8 5um 220X46mm HPLC COLUMN $24.95 Time Remaining: 4d 17h 48m |
 HPLC Column Waters SymmetryShield RP18 5um $198.00 Time Remaining: 28d 13h 58m Buy It Now for only: $198.00 |
 REGIS Val U PAK HP Column HPLC 731901 ODS 25cm x 46mm FREE SHIPPING $23.99 Time Remaining: 3d 11h 17m Buy It Now for only: $23.99 |
 Waters Delta Pak C18 HPLC Column 78x300mm 15um 011803 $78.00 Time Remaining: 4d 14h 49m Buy It Now for only: $119.00 |
 WATERS MILLIPORE HPLC TCM TEMPERATURE CONTROL WAT038000 CHM COLUMN HEATER MODULE $399.99 Time Remaining: 29d 10h 43m Buy It Now for only: $399.99 |
 Phenomenex Lichrospher RP18 5um HPLC Column 250 x 46mm $49.99 Time Remaining: 18d 10h 45m Buy It Now for only: $49.99 |
 PERKIN ELMER 0711 0231 220X46mm OD 300 Aquapore ODS 7um 300 HPLC COLUMN $49.95 Time Remaining: 4d 17h 48m |
 HPLC COLUMN GUARD CARTRIDGES Lot of 3 SEALED Adsorbosphere Alltech 28027 $24.95 Time Remaining: 15d 23h 55m Buy It Now for only: $24.95 |
 REGIS Val U PAK HP Column HPLC 731900 ODS 25cm x 46mm $23.99 Time Remaining: 3d 12h 55m Buy It Now for only: $23.99 |
 PERKIN ELMER 0711 0236 220X46mm OD 300 Aquapore ODS 7um 300 HPLC COLUMN $49.95 Time Remaining: 4d 17h 48m |
 TurboFlow HTLC C18 HPLC Column 505169 NEW $45.00 Time Remaining: 6d 8h 57m Buy It Now for only: $45.00 |
 HPLC column Merck Hibar RT Lichrospher 100 RP 8 5 um $49.00 Time Remaining: 9d 16m Buy It Now for only: $49.00 |
 WATERS 27477 Porasil Liquid Chromatography Column HPLC HEXANE $29.95 Time Remaining: 15h 5m Buy It Now for only: $89.00 |
 HPLC column Agilent Zorbax SB C8 46x150 mm $27.00 Time Remaining: 15d 16h 48m Buy It Now for only: $27.00 |
 ALLtech HPLC Column $88.35 Time Remaining: 15d 12h 25m Buy It Now for only: $88.35 |
 3 HIGGINS ANALYTICAL HF 2246 W185 HAISIL 300 C18 5um 220X46mm HPLC COLUMNS $49.95 Time Remaining: 4d 17h 48m |
 Phenomenex Luna 3u Phenyl Hexyl HPLC Column 150 x 46mm $49.99 Time Remaining: 18d 10h 46m Buy It Now for only: $49.99 |
 Bakerbond gold plated HPLC Column ABX PN 7271 07 New $29.99 Time Remaining: 7d 13h 34m Buy It Now for only: $29.99 |
 PERKIN ELMER 0711 0017 220X46mm RP 18 Spheri 5 C 18 5um HPLC COLUMN $49.95 Time Remaining: 4d 17h 48m |
 HPLC column Waters Protein pak 300 SW 300x75 mm 080013 gel filtration $117.00 Time Remaining: 10d 20h 40m Buy It Now for only: $117.00 |
 HPLC column YMC Jsphere ODS H80 150x46 mm S 4 um 80A $65.00 Time Remaining: 25d 16h 30m Buy It Now for only: $65.00 |
 Grace VYDAC HPLC Column 214TP C4 10x250mm 214TP1010 $58.00 Time Remaining: 5d 14h 25m Buy It Now for only: $89.00 |
 High pressure chromatography preparative column 30x250 mm prep HPLC $190.00 Time Remaining: 2d 17h 40m Buy It Now for only: $190.00 |
 NEW BERGER HPLC COLUMN SILICA 60A 6U 46 X 150mm SEALED $39.99 Time Remaining: 8d 18h 35m Buy It Now for only: $39.99 |
 Phenomenex PRODIGY 5u ODS2 Guard Column 30 x 460 mm 5 micron HPLC $14.95 Time Remaining: 15h 58m Buy It Now for only: $79.00 |
 HPLC COLUMN Agilent Zorbax ODS 46 x 250mm 5um $79.95 Time Remaining: 26d 15h 16m Buy It Now for only: $79.95 |
 HPLC column Supelco Supelcosil LC 18 DB 5um 46x250 mm $33.00 Time Remaining: 19d 16h 51m Buy It Now for only: $33.00 |
 HPLC Column Phenomenex CapCell Pak NH2 UG80 5um 20x35mm New $199.99 Time Remaining: 1d 1h 41m Buy It Now for only: $249.99 |
 NEW Berger Column DIOL 60A 6U 46 X 150mm HPLC Chromatography B1205 60762 $49.99 Time Remaining: 8d 18h 36m Buy It Now for only: $49.99 |
 HPLC COLUMN Waters Spherisorb ODS 1 46 x 300 mm NIB PSS839836 $219.95 Time Remaining: 7d 14h 59m Buy It Now for only: $219.95 |
 HPLC Column YMC Basic S 3 20x150 mm Part No BA99S031502WT 3 Available $79.99 Time Remaining: 1d 1h 14m Buy It Now for only: $89.99 |
 Zorbax Hypersil SI 46 X 200mm 5um HPLC Column T07 $80.95 Time Remaining: 13d 13h 44m Buy It Now for only: $80.95 |
 HPLC column Genesis AQ 4um FL10951E $50.00 Time Remaining: 5d 1h 6m Buy It Now for only: $50.00 |
 Hamilton PRPX400 HPLC Column $200.00 Time Remaining: 3d 8h 24m Buy It Now for only: $250.00 |
 Develosil 3 RP Aqueous C30 HPLC Column 150 x 46 mm $49.99 Time Remaining: 27d 20h 28m Buy It Now for only: $49.99 |
 New Waters HPLC guard cartridge column Guard PAK nova pak C18 15220 $47.00 Time Remaining: 15d 16h 15m Buy It Now for only: $47.00 |
 Waters HPLC Column XTerra MS P N 186000466 C18 5u 30x100 mm $69.99 Time Remaining: 1d 1h 27m Buy It Now for only: $79.99 |
 HPLC Column MetaChem Inertil 5um Phenyl 250 x 46mm NEW $99.99 Time Remaining: 10d 15h 11m Buy It Now for only: $99.99 |
 LudgerSep R1 HPLC Column Glycan LS R1 46x150 in Original Column Protector $299.99 Time Remaining: 22h 41m Buy It Now for only: $349.99 |
 HPLC COLUMN Agilent Lichrosorb RP 8 46 x 200 mm NIB 79915MO 174 $219.95 Time Remaining: 17d 16h 53m Buy It Now for only: $219.95 |
 Waters XBridge Shield RP18 HPLC Column 35 46 x 50mm $49.99 Time Remaining: 18d 10h 58m Buy It Now for only: $49.99 |
 YMC HPLC Column ODS A S 5 um 300A 20x250 mm Part No AA30S052502WT 2 Available $79.99 Time Remaining: 22h 39m Buy It Now for only: $89.99 |
 Waters Atlantis T3 HPLC Column 3u 46 x 75 mm $49.99 Time Remaining: 28d 13h 40m Buy It Now for only: $49.99 |
 HPLC column Hypersil Hypercare 5um 100mm $27.00 Time Remaining: 19d 16h 40m Buy It Now for only: $27.00 |
 ALLTECH 605NH C NH2 Column 5 Micron 25cm x 46mm HPLC $29.95 Time Remaining: 15h 32m Buy It Now for only: $104.00 |
 JTBAKER HPLC COLUMN 46 250 MM $15.00 Time Remaining: 13d 14h 32m Buy It Now for only: $15.00 |
 HPLC GUARD COLUMN TSKgel SW 75 mm x 75 cm Tosoh 05371 NIB $129.95 Time Remaining: 19h 38m Buy It Now for only: $129.95 |
 Cronus HPLC Column Lichrospher 100 NH2 5 um 25x04 cm 3 Available $104.99 Time Remaining: 22h 41m Buy It Now for only: $119.99 |
 Phenomenex Selectosil 00G 2002 E0 HPLC Column $99.95 Time Remaining: 27d 17h 9m Buy It Now for only: $99.95 |
 Bio Rad HPLC Guard Column for Bio Sil TSK Columns $17.50 Time Remaining: 19d 20h 13m Buy It Now for only: $17.50 |
 HPLC Column YMC ODS A S 5 um 30 nm 250x46 mm Part No AA30S05 2546WT 2 Available $79.99 Time Remaining: 1d 1h 25m Buy It Now for only: $89.99 |
 HPLC COLUMN Whatman Partisil 5 LOT OF 2 4215 001 NIB SEALED Chromatography $199.95 Time Remaining: 5d 17h 20m Buy It Now for only: $199.95 |
 HPLC column Agilent Zorbax SB C18 35um 21x75 mm $50.00 Time Remaining: 15d 21h 42m Buy It Now for only: $50.00 |
 TOSOH TSKgel DEAE 2SW Anion Exchange HPLC Column 5 m 46x250 mm Two in Lot $429.00 Time Remaining: 1d 1h 17m Buy It Now for only: $499.00 |
 MERCK 125911001 50 ID SELF PACKING DEVICE FOR 50MM ID PREPARATIVE HPLC COLUMNS $1,499.99 Time Remaining: 21d 11h 45m Buy It Now for only: $1,499.99 |
 New PE HPLC guard cartridge column Brownlee Procise cLC perkin elmer 401883 $33.00 Time Remaining: 15d 16h 2m Buy It Now for only: $33.00 |
 HPLC Column Agilent Zorbax 300SB C18 46x150mm 30x150mm New In The Box $279.99 Time Remaining: 22h 33m Buy It Now for only: $329.99 |
 Phenomenex Inertsil Phenyl HPLC Column 150 x 46mm 5u $49.99 Time Remaining: 21d 12h 57m Buy It Now for only: $49.99 |
 HPLC Column Michrom Bioresources 5u 200A Magic C18 $287.10 Time Remaining: 28d 13h 58m Buy It Now for only: $287.10 |
 Waters Xbridge HPLC Column C18 35um 46x50mm In Original Box 2 Available $99.99 Time Remaining: 1d 1h 30m Buy It Now for only: $119.99 |
 HPLC Column Alltech Hypersil BDS 5m C8 $247.50 Time Remaining: 28d 13h 58m Buy It Now for only: $247.50 |
 HPLC column Agilent Zorbax Eclipse XDB C18 C18 35um 46x150 mm RP18 $47.00 Time Remaining: 15d 16h 48m Buy It Now for only: $47.00 |
 HPLC Column Phenomenex Synergi Hydro RP 00F 4375 E0 80A 46x150 mm $89.00 Time Remaining: 22h 41m Buy It Now for only: $99.00 |
 HPLC Column Tosoh Bioscience TSKgel Phenyl 5PW RP $306.90 Time Remaining: 28d 13h 58m Buy It Now for only: $306.90 |
 Leco Model 521 Induction Furnace HPLC Column Heating Melting Furnace 120V 15A $495.00 Time Remaining: 21h 24m Buy It Now for only: $495.00 |
 Lot of 3 HPLC columns 250 mm x 46 mm $25.00 Time Remaining: 2d 16h 3m |
 Waters XBridge C18 HPLC Column 35 m 46 x 150mm $49.99 Time Remaining: 18d 10h 58m Buy It Now for only: $49.99 |
 Vydac Protein Peptide C18 Column HPLC 250x 22 mm 10 um preparative $370.00 Time Remaining: 13d 22h 31m Buy It Now for only: $370.00 |
 HPLC Column Supelco Ascentis RP Amide 3 um 3cm x 3mm 565310 U New in Box Sealed $219.99 Time Remaining: 22h 33m Buy It Now for only: $299.99 |
 New HPLC column Thermo guard cartridge 20x21 cat number 80000 506 $27.00 Time Remaining: 17d 14h 17m Buy It Now for only: $27.00 |
 HPLC Column YMC Jsphere ODS L80 250x46 mm S 4 um 80A $74.00 Time Remaining: 28d 17h 6m Buy It Now for only: $74.00 |
 YMC HPLC Column ODS A S 5 um 250x46 mm Part No AA30S052546WT 2 Available $79.99 Time Remaining: 22h 39m Buy It Now for only: $89.99 |
 New Waters HPLC guard cartridge column Guard PAK holder ferrule compression $53.00 Time Remaining: 21d 22h 8m Buy It Now for only: $53.00 |
 HPLC column Hibar LiChrospher 100 RP 8 5um RT 250 4 $33.00 Time Remaining: 17d 20h 58m Buy It Now for only: $33.00 |
 Thermo Electron Aquasil C18 HPLC Column $995.00 Time Remaining: 15h 10m |
 YMC PACK PRO C18 46 X 150MM HPLC COLUMN AS12S031546WT $149.99 Time Remaining: 15d 12h 51m Buy It Now for only: $149.99 |
 Alltech SPHERISORB ODS II HPLC COLUMN 3um 100 x 46mm w Waters Fitttings $24.95 Time Remaining: 25d 20h 46m Buy It Now for only: $24.95 |
 DIONEX STH 585 HPLC COLUMN OVEN with Controller $489.38 Time Remaining: 5d 10h 27m |
 Supelco Discovery C8 HPLC Column 59353 U40 $199.95 Time Remaining: 27d 17h 8m Buy It Now for only: $199.95 |
 HPLC COLUMN TSKGEL Super SW2000 46mm x 30 cm Tosoh 18674 NIB SEALED $749.95 Time Remaining: 10d 15h 59m Buy It Now for only: $749.95 |
 Beckman HPLC Column 5um 46mm x 15cm $39.99 Time Remaining: 6d 17m Buy It Now for only: $99.99 |
 Waters XTerra RP 8 HPLC Column 35u 46 x 50 mm $49.99 Time Remaining: 28d 13h 40m Buy It Now for only: $49.99 |
 HPLC column ACT Ace 3 C18 3um 100A 3x150 mm $89.00 Time Remaining: 22d 18h 21m Buy It Now for only: $89.00 |
 HPLC Column ES Industries LiChrospher NH2 46x250 mm 5 u 100A Cat 154221 LIS NH2 $79.99 Time Remaining: 6d 13h 23m Buy It Now for only: $99.99 |
 HPLC Column Phenomenex Sphereclone 5um ODS1 250 x 460 mm New $149.99 Time Remaining: 10d 14h 23m Buy It Now for only: $149.99 |
Hplc Column
Chemical detection of Aflatoxins in dairy products
High performance liquid chromatography
High performance liquid chromatography (HPLC) is a widely used analytical technique. It allows samples to be separated and their components determined.
The basic principle of HPLC is passing a sample through a column packed with tiny particles (usually less than 10um diameter) and applying high pressure to increase the resolution and speed of the technique.
There is solvent constantly flowing through the system. This solvent is called mobile phase. To ensure the solvent is constantly moving through the system a pump is employed. This gives the required pressure to allow mobile phase and sample to move through the column with ease and speed. The sample is injected into the mobile phase so it can proceed directly to the column. The sample comes out from the column (elutes) and proceeds to the detector. This monitors the refractive index difference between the pure mobile phase entering the column and the column elute.
The signal from the detector is sent to a data recorder. This measures the time of each component (retention time) and the size of the signal (peak area).
By injecting a known standard a retention time and peak area are achieved. If an unknown sample is then injected and peak obtained at the same retention time.
The peak area can be used to calculate the amount of standard material in the unknown by proportion.
HPLC is especially important for evaluation of non-volatile compounds, providing rapid determination of additives, contaminants and natural components of food products.
Mycotoxins present in food products
Mycotoxins are substances created by fungal secondary metabolic processes which are in connection with specific disorders in animals and humans. The action of toxicity in animals is as harmful as the fungal species which produce these compounds.
Aflatoxins in milk products
A notable example of mycotoxins is aflatoxin that usually brings about liver damage and cancer, reducing the milk production and inhibiting immune system.
Aflatoxin M1, is a toxin found in milk extracted from ingested aflatoxin. Identification of M1 is important since it is likely to be found in milk which is an essential source of nutrition for human.
There are significant regulations (throughout the world) concerning the presence of aflatoxin M1 in dairy products.
Afla M1 HPLC is a rapid aflatoxin test from which accurate numerical results can be achieved. Using monoclonal affinity chromatography, Afla M1 can isolate aflatoxin M1 from dairy products.
About the Author
Read more: Aflatoxins. Other subjects: Dayspring | Lynmar
What is the best HPLC column to purchase.?
It really depends on your application and what kind of separation you need. Lower analyte concentrations require higher resolution, which means purchasing a long column. Varian and Waters are 2 well-respected brands of HPLC columns. Here is an article that may help you with your decision.
http://www.biocompare.com/featurearticle.asp?articleid=711
HPLC Report: G8 HPLC Analyzer for High Performance Liquid Chromatography